WebHep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. [1] These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. [2] WebHep G2 [HEPG2] is a cell line exhibiting epithelial-like morphology that was isolated from a hepatocellular carcinoma of a 15-year-old, White, male youth with liver cancer. The cell line was deposited by the Wistar Institute and is a suitable transfection host. Hep G2 [HEPG2] HB-8065. Detailed product information. Expand all Collapse … ATCC Organoid Growth Kits make it easy to subculture and grow your patient … 0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium … Eagle's minimum essential medium modified to contain Earle's Balanced … ATCC is working to harmonize our Safety Data Sheet (SDS) distribution with … The Credible Conversations blog shares the science and innovation from inside …
Cell culture conundrum II: HepG2 cells grow slowly and forms …
WebHepG2 (ATCC® Cat. No. HB-8065™) is an epithelial, hepatocellular carcinoma cell line. ATCC achieved transfection efficiencies of approximately 95%, using the protocol … WebHere, we dynamically profile the uptake and excretion fluxes of a liver cancer cell line (HepG2) and use genome-scale metabolic modeling for in-depth analysis. We find that up to 30% of the glutamine is metabolized in the cytosol, primarily for nucleotide synthesis, producing cyto- solic glutamate. push buttons arduino
Hep G2 [HEPG2] - HB-8065 ATCC
Web结果提示，EGF可促进肝癌细胞系HepG2的增殖，并与其剂量有关。 2）逆转录：提取的总 RNA 加入 3 μL Olig（dT），70℃孵育5min后置于冰上5min。 依次加M-MLV逆转录酶 1μL、M-MLV Buffer 5 μL、Rnase inhibitor 1μL、Dntp 1.5μL，DEPC水加至 25μL，37℃水浴 60 min，合成 c-DNA。 1.2.2 MTT增殖指数 按每孔2×105个细胞接种于24孔板,分别以不同 … Hep G2 (or HepG2) is a human liver cancer cell line. Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. The cells se… Web3 okt. 2024 · Our HepG2 is growing at 37°C, 5%CO2 in a complete medium consist of the 500ml MEM alpha medium with GlutaMAX (life technologies 41090-028); 50ml FBS (no … push buttons for toilets