WebmAmetrine is potentially a near-ideal donor for pairing with an orange or red fluorescent protein (OFP or RFP) to produce a new FRET pair that would be spectrally compatible with the mTFP1-mCitrine pair for ratiometric imaging of dual FRET–based bio-sensors. To find an appropriate acceptor for mAmetrine, we WebPlasmid pMSCV-IRES-mAmetrine 1.1 FP from Dr. Dario Vignali's lab is published in Unpublished This plasmid is available through Addgene. Image: Illustrated plasmid map …

National Center for Biotechnology Information

WebNational Center for Biotechnology Information WebFigure 7a illustrates the concept: both targets contain a positive selection marker or a negative selection marker (hygtk or tkneo fusion gene, respectively), while the Flpo expression vector... how do you say lawn mower in spanish

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WebWe narrowed to 32 results for: mAmetrine. In Category. Catalog. Educational Resources; Plasmids. Filter By. Expression . Bacterial Expression (6) Insect Expression (1) Mammalian Expression (22) Yeast Expression (1) Vector Types . … WebThe first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular evolution led to the identification of FLPe which has a temperature optimum of 37 °C ( … WebOct 4, 2024 · As a successful example, we employed a new FRET pair mAmetrine (an LSS yellow fluorescence protein)/DY-547 (a cyanine derivative) to substitute CFP/fluorescein that we previously employed to study molecular interactions between cyclic nucleotide-binding domains and cyclic nucleotides. how do you say lay down in russian