WebThe Duolink proximity ligation assay was purchased from Sigma-Aldrich and performed in a format suitable for the detection of single proteins and protein-protein interactions, as described in detail previously [30,53,54]. In brief, hVSMC and mouse VSMC were fixed on eight well tissue culture slides (Nunc). WebMar 31, 2016 · View Full Report Card. Fawn Creek Township is located in Kansas with a population of 1,618. Fawn Creek Township is in Montgomery County. Living in Fawn …
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WebThe Duolink® PLA signal is recognized as discrete fluorescent spots in various locations of the studied cells (see Figure 1A). Individual signals are of sub-micrometer size and ... Protein detection using proximity-dependent DNA ligation assays. Nat Biotechnol, 20, 473-77 (2002). Figure 3. Image analysis using imaging software. The DAPI ... WebFeb 19, 2024 · 2.3 Proximity Ligation Assay 1. Duolink In Situ Detection Reagents kit in Orange (Cy3) (Sigma). Other options/fluorophores are available. The kit contains 5× ligation stock, 1× ligase, 5× amplification stock, 1× polymerase. 2. Wash Buffer A: Tris-buffered saline solution with 0.05% Tween 20, pH 7.4. flysheet tent
Duolink PLA Fluorescence Protocol - Sigma-Aldrich
WebApr 11, 2024 · DuoLink proximity ligation assay. To visualize interactions between p38 MAPK and GSK3β, a proximity ligation assay (PLA) was performed using the Duolink® Kit (Sigma-Aldrich®) according to the manufacturer’s instructions. The first step was as described above for the immunofluorescence experiments with two primary antibodies … WebAug 6, 2013 · The following PLA components were purchased from Olink (Uppsala, Sweden): Duolink PLA probe anti-rabbit PLUS, Duolink PLA probe anti-mouse MINUS and Duolink in situ Detection Reagent Red. PLAs were performed according to the manufacturer. ... In situ proximity ligation assays (PLA) on vertical sections through … WebIn situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols. flyshen