WebPre-Clear The Lysate (Optional) Pre-clearing is a step that decreases binding of non-specific proteins, lipids, carbohydrates, or nucleic acids. This step is performed by … WebSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Protocol Specificity / Sensitivity Transferrin (E7F4T) Rabbit mAb recognizes endogenous levels of total transferrin protein. Species Reactivity: Human Source / Purification
Immunoprecipitation Protocol (Magnetic Beads) - Cell Signaling Technology
WebMay 20, 2024 · Supernatants (10 μL in 3-fold final dilution at 0.217–0.446 mg/mL) were preincubated in the absence or presence of 2 µM Inhibitor-2 protein at 30 °C for 1 min, then incubated with 1 µM 32 P-labeled 20 kDa light chain of gizzard myosin ( 32 P-MLC20) substrate for 30 s. WebMethod Pre-clearing of the lysate (optional step) Take 100 µl of lysate and add antibody of the same species and isotype as the antibody to be used for the immunoprecipitation procedure at a 1/20 dilution. Incubate on ice for 1 hr. Thoroughly resuspend the SureBeads Magnetic Beads in their solution and transfer 100 µl (1 mg at fchd york city
Transferrin (E7F4T) Rabbit mAb Cell Signaling Technology
WebAug 28, 2024 · Barnase catalyses the cleavage of single-stranded RNA through hydrolysis of the phosphodiester bonds at GpN sites, with a higher rate for GpG and GpA sites. The activity was reported in the pH range of 7.5 to 9.3 with a maximum at 8.5 [ 2 ]. WebJun 14, 2007 · Preparation of a cleared lysate. 1. Harvest the 100 ml culture by centrifugation (4,500g, 15 min, 4 °C) and resuspend cells with 1 ml Buffer W. Add … WebMay 26, 2006 · Membrane cleared cell lysates were harvested from human embryonic kidney (HEK) 293 cells transfected with pSFFV empty vector (V), pSFFV-FLAG-YopJ (J), or pSFFV-FLAG-YopJ-C/A (C/A) ( 10 ). ( A) Lysates were incubated with purified B-Raf for 10 min at 37°C, followed by immunoblotting with antibody to phospho-ERK. frits coers